Structure and properties of seminal ribonuclease.

نویسندگان

  • E Leone
  • G D'Alessio
چکیده

Brand, R. C. & Planta, R. J. (1975) Mol. Biol. Rep. 2, 321-325 Klootwijk, J. & Planta, R. J. (1973) Eur. J. Biochem. 39, 325-333 Maden, B. E. H. & Salim, M. (1974) J. Mol. Biol. 88, 133-164 Maden, B. E. H., Forbes, J., De Jonge, P. & Klootwijk, J. (1975) FEBSLerr. 59,60-63 Perry, R. P. (1976) Annu. Rev. Biochem. 45, 605-629 Planta, R. J., Van den Bos, R. C. & Klootwijk, J. (1972) in Ribosomes: Structure, Function and Biogenesis (Bloemendal, H. & Planta, R. J., eds.), pp. 183-196, North-Holland, Amsterdam Retd, J. & Planta, R. J. (1972) Biochim. Biophys. Actu 281, 299-309 Retkl, J. & Van Keulen, H. (1975) Eur. J. Biochem. 58, 51-57 Rubin, G . M. (1973) J. Biol. Chem. 248,3860-3875 Trapman, J. & Planta, R. J. (1975) Biochim. Biophys. Actu 414, 115-125 Trapman, J., De Jonge, P. & Planta, R. J. (1975) FEBS Lett. 57, 26-30 Van den Bos, R. C., Retbl, J. & Planta, R. J. (1971) Biochim. Biophys. Acta 232,494-508 Van Keulen, H., Planta, R. J. & RetM, J. (1975) Biochim. Biophys. Actu 395, 179-190

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Bovine seminal ribonuclease (BS-RNase) is a homologue of RNase A with special biological properties, including potent immunosuppressive activity. A mutant BS-RNase was created in which His-119, the active-site residue that acts as a general acid during catalysis, was changed to an aspartic acid. H119D BS-RNase formed a dimer with quaternary structure similar to that of the wild-type enzyme but ...

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Bovine seminal ribonuclease (BS-RNase), a dimeric homolog of bovine pancreatic ribonuclease A (RNase A), is toxic to mammalian cells. In contrast to dimeric BS-RNase, a monomeric BS-RNase and RNase A are not cytotoxic and are bound tightly by cytosolic ribonuclease inhibitor. To elucidate the mechanism of ribonuclease cytotoxicity, we constructed a series of hybrid and semisynthetic enzymes and...

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عنوان ژورنال:
  • Biochemical Society transactions

دوره 5 2  شماره 

صفحات  -

تاریخ انتشار 1977